Publications

2016

  1. Acquasaliente L, Peterle D, Tescari S, Pozzi N, Pengo V, De Filippis V. Molecular mapping of α-thrombin (αT)/β2-glycoprotein I (β2GpI) interaction reveals how β2GpI affects αT functions. Biochem J. 2016 Dec 15;473(24):4629-4650.
  2. Eickhoff CS, Zhang X, Vasconcelos JR, Motz RG, Sullivan NL, O’Shea K, Pozzi N, Gohara DW, Blase JR, Di Cera E, Hoft DF. Costimulatory Effects of an Immunodominant Parasite Antigen Paradoxically Prevent Induction of Optimal CD8 T Cell Protective Immunity. PLoS Pathog. 2016 Sep 19;12(9)
  3. Pozzi N, Bystranowska D, Zuo X, Di Cera E. Structural Architecture of Prothrombin in Solution Revealed by Single Molecule Spectroscopy. J Biol Chem. 2016 Aug 26;291(35):18107-16.
  4. Pozzi N, Di Cera E. Dual effect of histone H4 on prothrombin activation. J Thromb Haemost. 2016 Sep;14(9):1814-8.
  5. Pozzi N, Zerbetto M, Acquasaliente L, Tescari S, Frezzato D, Polimeno A, Gohara DW, Di Cera E, De Filippis V. Loop Electrostatics Asymmetry Modulates the Preexisting Conformational Equilibrium in Thrombin. Biochemistry. 2016 Jul 19;55(28):3984-94.
  6. Pozzi N, Chen Z, Di Cera E. How the Linker Connecting the Two Kringles Influences Activation and Conformational Plasticity of Prothrombin. J Biol Chem. 2016 Mar 18;291(12):6071-82.

2015

  1. Pelc LA, Chen Z, Gohara DW, Vogt AD, Pozzi N, Di Cera E. Why ser and not thr brokers catalysis in the trypsin fold. Biochemistry. 2015 Feb 24;54(7):1457-64.
  2. Wood DC, Pelc LA, Pozzi N, Wallisch M, Verbout NG, Tucker EI, Gruber A, Di Cera E. WEDGE: an anticoagulant thrombin mutant produced by autoactivation. J Thromb Haemost. 2015 Jan;13(1):111-4.

2014

  1. Pozzi N, Di Cera E. Prothrombin structure: unanticipated features and opportunities. Expert Rev Proteomics. 2014 Dec;11(6):653-5.
  2. Pozzi N, Chen Z, Pelc LA, Shropshire DB, Di Cera E. The linker connecting the two kringles plays a key role in prothrombin activation. Proc Natl Acad Sci USA. 2014 May 27;111(21):7630-5.
  3. Vogt AD, Pozzi N, Chen Z, Di Cera E. Essential role of conformational selection in ligand binding. Biophys Chem. 2014 Feb;186C:13-21.

2013

  1. Barranco-Medina S*, Pozzi N*, Vogt AD, Di Cera E. Histone H4 promotes prothrombin autoactivation. J Biol Chem. 2013 Dec 13;288(50):35749-57.
  2. Pozzi N, Chen Z, Gohara DW, Niu W, Heyduk T, Di Cera E. Crystal structure of prothrombin reveals conformational flexibility and mechanism of activation. J. Biol Chem. 2013 Aug 2;288(31):22734-44.
  3. Pozzi N, Acquasaliente L, Frasson R, Cristiani A, Moro S, Banzato A, Pengo V, Scaglione GL, Arcovito A, De Cristofaro R, De Filippis V. β2-Glycoprotein I binds to thrombin and selectively inhibits the enzyme procoagulant functions. J Thromb Haemost. 2013 Jun;11(6):1093-102.
  4. Pozzi N, Chen Z, Zapata F, Niu W, Barranco-Medina S, Pelc LA, Di Cera E. Autoactivation of thrombin precursors. J Biol Chem. 2013 Apr 19;288(16):11601-10.

2012

  1. Pozzi N, Vogt AD, Gohara DW, Di Cera E. Conformational selection in trypsin-like proteases. Curr Opin Struct Biol. 2012 Aug;22(4):421-31.
  2. Pozzi N*, Barranco-Medina S*, Chen Z, Di Cera E. Exposure of R169 controls protein C activation and autoactivation. Blood. 2012 Jul 19;120(3):664-70.
  3. De Filippis V, Lancellotti S, Maset F, Spolaore B, Pozzi N, Gambaro G, Oggianu L, Calò LA, De Cristofaro R. Oxidation of Met1606 in von Willebrand factor is a risk factor for thrombotic and septic complications in chronic renal failure. Biochem J. 2012 Mar 1;442(2):423-32.
  4. Pozzi N, Lancellotti S, De Cristofaro R, De Filippis V. Modeling ADAMTS13-von Willebrand factor interaction: Implications for oxidative stress-related cardiovascular diseases and type 2A von Willebrand disease. Biophys Chem. 2012 Jan;160(1):1-11.

2011

  1. Banzato A, Pozzi N, Frasson R, De Filippis V, Ruffatti A, Bison E, Padayattil SJ, Denas G, Pengo V. Antibodies to Domain I of β(2)Glycoprotein I are in close relation to patients risk categories in Antiphospholipid Syndrome (APS). Thromb Res. 2011 Dec;128(6):583-6.
  2. Pozzi N, Chen Z, Zapata F, Pelc LA, Barranco-Medina S, Di Cera E. Crystal structures of prethrombin-2 reveal alternative conformations under identical solution conditions and the mechanism of zymogen activation. Biochemistry. 2011 Nov 29;50(47):10195-202.
  3. Pozzi N, Chen R, Chen Z, Bah A, Di Cera E. Rigidification of the autolysis loop enhances Na(+) binding to thrombin. Biophys Chem. 2011 Nov;159(1):6-13.
  4. Vottariello F, Giacomelli E, Frasson R, Pozzi N, De Filippis V, Gotte G. RNase A oligomerization through 3D domain swapping is favoured by a residue located far from the swapping domains. Biochimie. 2011 Oct;93(10):1846-57.
  5. Lancellotti S, De Filippis V, Pozzi N, Oggianu L, Rutella S, Scaglione GL, Maset F, Peyvandi F, Mannucci PM, De Cristofaro R. Oxidized von Willebrand factor is efficiently cleaved by serine proteases from primary granules of leukocytes: divergence from ADAMTS-13. J Thromb Haemost. 2011 Aug;9(8):1620-7
  6. Niu W, Chen Z, Gandhi PS, Vogt AD, Pozzi N, Pelc LA, Zapata F, Di Cera E. Crystallographic and kinetic evidence of allostery in a trypsin-like protease. Biochemistry. 2011 Jul 26;50(29):6301-7.

2010

  1. Rana S, Pozzi N, Pelc LA, Di Cera E. Redesigning allosteric activation in an enzyme. Proc Natl Acad Sci U S A. 2011 Mar 29;108(13):5221-5.
  2. Li W, Johnson DJ, Adams TE, Pozzi N, De Filippis V, Huntington JA. Thrombin inhibition by serpins disrupts exosite II. J Biol Chem. 2010 Dec 3;285(49):38621-9.
  3. Pozzi N, Banzato A, Bettin S, Bison E, Pengo V, De Filippis V. Chemical synthesis and characterization of wild-type and biotinylated N-terminal domain 1-64 of beta2-glycoprotein I. Protein Sci. 2010 May;19(5):1065-78.
  4. Lancellotti S, De Filippis V, Pozzi N, Peyvandi F, Palla R, Rocca B, Rutella S, Pitocco D, Mannucci PM, De Cristofaro R. Formation of methionine sulfoxide by peroxynitrite at position 1606 of von Willebrand factor inhibits its cleavage by ADAMTS-13: A new prothrombotic mechanism in diseases associated with oxidative stress. Free Radic Biol Med. 2010 Feb 1;48(3):446-56.

2009

  1. Arcone R, Chinali A, Pozzi N, Parafati M, Maset F, Pietropaolo C, De Filippis V. Conformational and biochemical characterization of a biologically active rat recombinant Protease Nexin-1 expressed in E. coli. Biochim Biophys Acta. 2009 Apr;1794(4):602-14.

2008

  1. Lancellotti S, Rutella S, De Filippis V, Pozzi N, Rocca B, De Cristofaro R. Fibrinogen-elongated gamma chain inhibits thrombin-induced platelet response, hindering the interaction with different receptors. J Biol Chem. 2008 Oct 31;283(44):30193-204.