Michelle Pherson Publishes PRC1 Research

PRC1 modifies transcription of active genes in BG3 cells.

(A) Effects of Ph depletion (iPh) on transcript accumulation (total RNA-seq) and transcription (3′ NT-seq). The left dot plot shows the log2 fold change in RNA levels from expressed genes upon Ph depletion versus the log2 RNA level in the mock-treated control cells using total RNA-seq. Red points are genes in which the change in total RNA levels caused by Ph depletion is significant at a false discovery rate of ≤5% (q ≤ 0.05). The right dot plot shows the same by 3′ NT-seq. Orange indicates where the change is statistically significant (q ≤ 0.05). (B) Both dot plots show the fold change in RNA accumulation (total RNA) versus that in transcription (3′ NT-seq) upon Ph depletion (iPh). The two graphs are identical except that the left shows which changes are significant by total RNA-seq (red dots) and the right shows those significant by 3′ NT-seq (orange dots).
PRC1 modifies transcription of active genes in BG3 cells. (A) Effects of Ph depletion (iPh) on transcript accumulation (total RNA-seq) and transcription (3′ NT-seq). The left dot plot shows the log2 fold change in RNA levels from expressed genes upon Ph depletion versus the log2 RNA level in the mock-treated control cells using total RNA-seq. Red points are genes in which the change in total RNA levels caused by Ph depletion is significant at a false discovery rate of ≤5% (q ≤ 0.05). The right dot plot shows the same by 3′ NT-seq. Orange indicates where the change is statistically significant (q ≤ 0.05). (B) Both dot plots show the fold change in RNA accumulation (total RNA) versus that in transcription (3′ NT-seq) upon Ph depletion (iPh). The two graphs are identical except that the left shows which changes are significant by total RNA-seq (red dots) and the right shows those significant by 3′ NT-seq (orange dots).

Michelle Pherson, Graduate Student in the lab of Dale Dorsett, Ph.D. recently published a paper in Science Advances detailing her research on Polycomb repressive complex 1 (PRC1) and its role in active gene transcription.

PRC1 is a protein complex important for development, and is also involved in cancer and genetic diseases. Using genome-wide RNA sequencing and chromatin immunoprecipitation, Michelle showed that PRC1 directly affects transcription of active genes, indicating a much larger role in gene regulation that previously thought.

Department of Biochemistry and Molecular Biology
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