Edward A. Doisy Department of
Biochemistry and Molecular Biology

Previous MaD for Single Molecules Workshops



2013


The first annual workshop took place on Thursday, December 12, 2013. Approximately 75 researchers attended the workshop, which took place at the Edward Doisy Research Center at Saint Louis University.





Workshop Schedule


10:00 am:
10:30-11:00 am:
11:00-11:30 am:
11:30 am-12:00 pm:
12:00-1:15 pm:

1:15-1:45 pm:

1:45-2:15 pm:
2:15-2:30 pm:
2:30-5:00 pm:
Registration
Welcome from PicoQuant GmbH and Saint Louis University
"State-of-the-art, time-resolved single molecule measurements (PIE-smFRET, FCS, FLIM) with the MicroTime 200"

Steffen Rüttinger, Ph.D.
PicoQuant GmbH
"Dynamics of SSB proteins on single stranded DNA"

Timothy Lohman, Ph.D.
Washington University
Group Picture and Lunch Break

"Observation of ribosome intersubunit motion and L1 stalk conformational dynamics by smFRET"

Peter Cornish, Ph.D.
University of Missouri-Columbia

"Role of conformational flexibility in prothrombin function"

Nicola Pozzi, Ph.D.
Saint Louis University
Closing remarks
Instrument demonstration and open discussion

A printable schedule may be viewed here: Workshop Flyer.


2014


The second annual workshop took place on Thursday, December 11, 2014. Approximately 100 researchers attended the workshop, which took place at Il Monastero at Saint Louis University.





Workshop Schedule


9:00 am:
9:30-9:45 am:
9:45-10:30 am:
10:30-11:15 am:
11:15 am-12:00 pm:
12:00-1:15 pm:

1:15-2:00 pm:
2:00 pm:
3:30 pm:
Registration and coffee
Welcome from PicoQuant GmbH and Saint Louis University
"Small quantum dots for nanometer accuracy and resolution
on molecular motors and living neurons"

Paul Selvin, Ph.D.
University of Illinois-Urbana
"State-of-the-art time-resolved single molecule measurements
(PIE-smFRET, FCS, FLIM) with the MicroTime 200"

Volker Buschmann, Ph.D.
PicoQuant GmbH
"Visualizing protein function at the single molecule level"

David Millar, Ph.D.
Scripps Research Institute
Group Picture and Lunch Break

"Mechanisms of eukaryotic transcription initiation"

Eric Galburt, Ph.D.
Washington University
Closing remarks
Optional tour of laboratory and MicroTime 200 demonstration
(at the Doisy Research Center, 1100 S. Grand Blvd.)

A printable schedule may be viewed here: Workshop Flyer.


2015


The third annual workshop took place on Friday, December 11, 2015. Approximately 85 researchers attended the workshop, which took place at Il Monastero at Saint Louis University.





Workshop Schedule


10:00-10:25 am:
10:25-10:30 am:
10:30-11:30 am:
11:30 am-12:30 pm:
12:30-1:30 pm:

1:30-2:30 pm:
2:30-2:45 pm:

2:45-4:05 pm:
4:30 pm:
Registration and coffee
Welcome
"Transition paths in protein folding: Single molecule experiments,
theory, and simulations"

William Eaton, M.D., Ph.D.
NIDDK, NIH
"Fluorescence Correlation Spectroscopy and its many descendents: Precursors and complements to single molecule methods"

Elliot Elson, Ph.D.
Washington University
Group Picture and Lunch Break

Session 1: Student and Postdoc Talks


1:30-1:50 pm: Nicola Pozzi, Ph.D., Saint Louis University

"Structural characterization of multi-domain flexible
proteins using sm-PIE-FRET"




1:50-2:10 pm: Eric Tomko, Ph.D., Washington University

"TFIIH generates a five basepair open complex during
RNAP II transcription initiation"




2:10-2:30 pm: Saurabh Pratap Singh, Ph.D., Washington University

"Unwinding of DNA by individual Pif1 monomer"

Coffee Break

Session 2: Student and Postdoc Talks


2:45-3:05 pm: Josh Sokoloski, Ph.D., Washington University

"Translocase-induced reorganization of single-stranded
DNA binding proteins along single-stranded DNA"




3:05-3:25 pm: Drew Menke, University of Missouri-Columbia

"Examining ribosome dynamics using smFRET"




3:25-3:45 pm: Xinyue Zhang, Ph.D., University of Missouri-Columbia

"Mimicking ribosomal vectorial unfolding of RNA pseudoknot
in a protein channel"




Happy Hour at The Field House Bar & Grill
510 N. Theresa Blvd.















A printable schedule may be viewed here: Workshop Flyer.


2016


The fourth annual workshop took place on Friday, December 2, 2016. Approximately 55 researchers attended the workshop, which took place in the Allied Health Building Multipurpose Room at Saint Louis University.





Workshop Schedule


9:30-9:55 am:
9:55-10:00 am:
10:00-11:00 am:
11:00 am-11:50 am:
11:50 am-1:00 pm:
1:00-1:50 pm:
1:50-2:40 pm:
2:40-2:50 pm:
2:50-4:00 pm:
4:00-5:00 pm:
Registration and coffee
Welcome
"Division of labor among the subunits of a highly coordinated
ring ATPase"

Carlos Bustamante, Ph.D.
University of California-Berkeley
"Mechanosensing by myosin molecular motors"

Michael Greenberg, Ph.D.
Washington University
Group Picture and Lunch Break
"Single-molecule DNA fiber analysis of replication fork dynamics
and perturbation in living cells"

Alessandro Vindigni, Ph.D.
Saint Louis University
"Time-resolved single-molecule STED microscopy and spectroscopy"

Olaf Schulz, Ph.D.
PicoQuant GmbH
Coffee Break
Short Talks


2:50-3:20 pm: "Single-molecule of intrisically disordered proteins"

Andrea Soranno, Ph.D.
Washington University




3:20-3:40 pm: "Translation termination and ribosome rescue:

Effects of RF1 and YaeJ (ArfA) on ribosome dynamics"

Widler Casy
Univeristy of Missouri-Columbia




3:40-4:00 pm: "Fishing without line: Interference-free detection

of genetic biomarkers using nano pore dielectophoresis"

Kai Tian
University of Missouri-Columbia




Optional tour of the lab and MicroTime demonstration.









A printable schedule may be viewed here: Workshop Flyer.