Selected publications (lab members in cyan)

 2024

  1. Ponzar N*, Chinnaraj M*, Pagotto A, De Filippis V, Flaumenhaft R, Pozzi N. Mechanistic basis of activation and inhibition of Protein Disulfide Isomerase by allosteric antithrombotic compounds. J Thromb Haemost. 2024 Oct 23:S1538-7836(24)00625-1. doi: 10.1016/j.jtha.2024.09.036. PMID: 39454880 

  2. Pozzi N, Pontara E, Kumar S, Pengo V. Understanding the difference between type I and type II antiprothrombin antibodies and their effect on activated protein C resistance. J Thromb Haemost. 2024 Oct;22(10):2935-2938. doi: 10.1016/j.jtha.2024.07.005. PMID: 39029741

  3. Ward C, Curry N, El-Ekiaby M, Jurk K, Versteeg HH, Keragala C, Burstyn-Cohen T, Antoniak S, Suzuki Y, Baker RI, Christophe O, Revel-Vilk S, Hart A, Deppermann C, Tran H, Pozzi N, Kahr WHA, Grover SP, Wenzel P, Brown AC, Oury C, Shea SM, Fredenburgh J, Passam FH, Winearls J, Moore HB, Tole S, Merriman E, Barnes GD, Liu ZL, Sholzberg M. Illustrated State-of-the-Art Capsules of the ISTH 2024 Congress. Res Pract Thromb Haemost. 2024 May 3;8(4):102432. doi: 10.1016/j.rpth.2024.102432. eCollection 2024 May.PMID: 38854821

  4. Hasdemir HS, Pozzi N, Tajkhorshid E. Atomistic characterization of β2-glycoprotein I domain V interaction with anionic membranes. J Thromb Haemost. 2024 Jul 22:S1538-7836(24)00427-6. doi: 10.1016/j.jtha.2024.07.010. PMID: 39047943 

  5. Kumar S, Summers B, Basore K, Pengo V, Flaumenhaft R, Pozzi N. Cryo-EM structure and functional basis of prothrombin recognition by a Type-I anti-prothrombin antiphospholipid antibody. Blood. 2024 Feb 16:blood.2023022942. doi: 10.1182/blood.2023022942. *Cover and Commentary-> Urbanus R.T. Prothrombin forced into an awkward position. Blood (2024) 143 (19): 1888–1890. https://doi.org/10.1182/blood.202402424

2023

  1. Taylor A, Kumar S, Pozzi N. Forecasting the Future of Antiphospholipid Syndrome: Prospects and Challenges. Mo Med. 2023 Sep-Oct;120(5):359-366.

  2. Kumar S, Wulf J 2nd, Basore K, Pozzi N. Structural analyses of β2-glycoprotein I: is there a circular conformation? J Thromb Haemost. 2023 Aug 1:S1538-7836(23)00579-2. *Commentary-> Lancellotti S, Sacco M., De Cristofaro R. Commentary on “Structural analyses of β2-glycoprotein I: is there a circular conformation?” J Thromb Haemost. 2023 Dec;21(12):3354-3356.

  3. Java A, Atkinson J, Hu Z, Pozzi N. Mutations in atypical hemolytic uremic syndrome provide evidence for the role of calcium in complement factor I. Blood. 2023 Aug 10;142(6):607-610.

  4. Ponzar N, Pozzi N. Probing the conformational dynamics of thiol-isomerases using non-canonical amino acids and single-molecule FRET.  Methods. 2023 Apr 15;214:8-17.

  5. Yang M, Chiu J, Scartelli C, Ponzar N, Patel S, Patel A, Ferreira RB, Keyes RF, Carroll K, Pozzi N, Hogg PJ, Smith BC, Flaumenhaft R. Sulfenylation links oxidative stress to protein disulfide isomerase oxidase activity and thrombus formation. J. Thromb Haemost. 2023 Apr 8:S1538-7836(23)00268-4.

2022

  1. Chinnaraj M, Flaumenhaft R, Pozzi N. Reduction of protein disulfide isomerase results in open conformations and stimulates dynamic exchange between structural ensembles. J Biol Chem. 2022 Jun 30;298(8):102217.

2021

  1. Chinnaraj M, Pengo V, Pozzi N. A Novel ELISA Assay for the Detection of Anti-Prothrombin Antibodies in Antiphospholipid Syndrome Patients at High Risk of Thrombosis. Front Immunol. 2021 Sep 8;12:741589.

  2.  Kumar S*, Chinnaraj M*, Planer W, Zuo X, Macor P, Tedesco F, Pozzi N. An allosteric redox switch in domain V of β2-glycoprotein I controls membrane binding and anti-domain I autoantibody recognition. J Biol Chem. 2021 Aug;297(2):100890. (* equal contribution)

  3. Chinnaraj M, Barrios DA, Frieden C, Heyduk T, Flaumenhaft R, Pozzi N. Bioorthogonal Chemistry Enables Single-Molecule FRET Measurements of Catalytically Active Protein Disulfide Isomerase. Chembiochem. 2021 Jan 5;22(1):134-138.

2020

  1.  Ruben E*, Planer W*, Chinnaraj M, Chen Z, Zuo X, Pengo V, De Filippis V, Alluri RK, McCrae KR, Macor P, Tedesco F, Pozzi N. The J-elongated conformation of β2-glycoprotein I predominates in solution: implications for our understanding of antiphospholipid syndrome. J Biol Chem. 2020 Jul 31;295(31):10794 (* equal contribution).

2019

  1. Chinnaraj M, Planer W, Pengo V, Pozzi N. Discovery and characterization of 2 novel subpopulations of aPS/PT antibodies in patients at high risk of thrombosis. Blood Adv. 2019 Jun 11;3(11):1738-1749.

2018

  1. Chinnaraj M, Planer W, Pozzi N. Structure of Coagulation Factor II: Molecular Mechanism of Thrombin Generation and Development of Next-Generation Anticoagulants. Front Med (Lausanne). 2018;5:281.

  2. Chinnaraj M, Chen Z, Pelc LA, Grese Z, Bystranowska D, Di Cera E, Pozzi N. Structure of prothrombin in the closed form reveals new details on the mechanism of activation. Sci Rep. 2018 Feb 13;8(1):2945

2016

  1. Pozzi N, Bystranowska D, Zuo X, Di Cera E. Structural Architecture of Prothrombin in Solution Revealed by Single Molecule Spectroscopy. J Biol Chem. 2016 Aug 26;291(35):18107-16. 

Patents

  1. Thrombin-thrombomodulin fusion proteins as a powerful anticoagulant. US20190153419A1, WO2016176440A2.

  2. Expression of thrombin variants. WO2012088220A33