Services

Single cell sequencing can address tissue specific and/or cell type specific biological questions by examining RNA expression or epigenetic information on the single cell level. The Genomics Core houses a 10X Genomics Chromium iX, which has the capacity to prepare GEMs for all standard 10X Genomics single cell pipelines:

• 3′ scRNAseq
• single cell immune profiling
• fixed RNA single cell gene expression Flex
• scATACseq
• scMultiome

The core will take your single cell experiments from cells to final library, which can be submitted to external sequencing providers. We can help with the submission process and also assist you with raw data transfer, processing, and analysis.

The key to high quality genomic data is high quality input materials and output libraries. To measure total RNA, cDNA, and DNA libraries, the Core facility has two instruments:

• Qubit fluorimeter: quantify RNA and DNA concentration
• Agilent Bioanalyzer: quantify and measure size distribution of RNA and DNA

We can help with data processing and custom analysis for most genomic data, including bulk and single cell RNAseq, ATAC-seq, Cut&Run, ChIP-seq, and Cut&Tag. We are happy to discuss projects to help with your data analysis as well as provid assistance with figure generation and GEO data uploads.