Heyduk Lab Studies RNA Polymerase Escape

The Heyduk Lab published a paper in Nucleic Acids Research entitled “DNA template sequence control of bacterial RNA polymerase escape from the promoter.” The article details their studies on how RNA polymerase and the promotor interact to transition from initiation to elongation during DNA transcription. They found that Initially Transcribed Sequences or ITS control promotor escape in various ways. Using Next Generation Sequencing (NGS), they studied the escape properties of thousands of ITS variants to determine how each affects the escape of RNA polymerase from the promotor.

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Figure 1. (A) Example sequence of a promoter construct used for experiments in panel B. The example shown is for λPR promoter with λPR ITS.

Department of Biochemistry and Molecular Biology