David Ford, Ph.D.
Professor and Director of the CCR
Compared with subjects who never developed ARDS, those who developed ARDS within the first 6 days of sepsis have higher circulating levels of free 2-ClPA and free 2-ClSA on the day of ICU admission.
Myeloperoxidase-derived 2-chlorofatty acids contribute to human sepsis mortality via acute respiratory distress syndrome. Meyer NJ, Reilly JP, Feng R, Christie JD, Hazen SL, Albert CJ, Franke JD, Hartman CL, McHowat J, Ford DA. J Clin Invest Insight 2(23):e96432, 2017 (PMID: 29212955).
Human coronary artery endothelial cells were treated with the click-chemistry analog of 2-chloropalmitic acid. The click analog was clicked to an azide-TAMRA (red) to visualize the localization of 2-chloropalmitic acid. This co-localized with P-selectin and von Willebrand Factor (VWF) indicating the selective localization of 2-chloropalmitic acid with the Weibel Palade bodies.
2-Chlorofatty acids induce Weibel-Palade body mobilization. Hartman CL, Duerr MA, Albert CJ, Neumann WL, McHowat J, Ford DA. J Lipid Res 59(1):113-122, 2018 (PMID: 29167411).
Neutrophil extracellular traps (green strands/nets in image under 2-ClPA, 2-chloropalmitic acid) are selectively produced compared to palmitic acid. PMA (phorbol myristate acetate) is shown as a positive control for NET formation.
2-Chlorofatty acids: Lipid mediators of neutrophil extracellular trap formation. Palladino END, Katunga LA, Kolar GR, Ford DA. J Lipid Res 59(8):1424-1432, 2018 (PMID: 29739865)